Adequating the Genetic Transformation Protocol via Agrobacterium tumefaciens of Wheat Genotypes Recommended for the Brazilian Cerrado

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Wheat production in Brazil has been insufficient to supply the internal market's demand, being thus necessary to increase production, productivity levels as well as cultivated areas. The planting process in the Cerrado region in Central Brazil is seen as an alternative to decrease wheat production's deficit in the country once the region offers advantages such as high luminosity, dry climate, higher possibility of disease control, and industrial quality grains. The types of cultivation in the region are dryland farming and irrigated systems, the first presenting the greatest number of potential areas in Cerrado. The dry weather presents advantages for systems of irrigated cultivation, but hinders the cultivation through dryland farming, once the lack of rain during the grain filling period compromises productivity. In addition to that, the availability of recommended cultivars for dryland farming is limited. A more competitive cultivation of wheat in dryland farming systems would be important in productive systems of grains in Cerrado regions, as an alternative of diversification of complementarity to the cultivation of double crop corn, dryland farming beans and sorghum. The use of transgenic varieties is a biotechnological tool currently available which can help genetic improvement programs in a singular manner, especially in situations of difficult solution with the use of conventional techniques, such as the tolerance to droughts and resistance to the rice blast fungus (Magnaporthe grisea). Dominating the transgenic technique may help the introduction of genes in wheat genotypes without carrying its surrounding genes, or genes of other species, in addition to artificially modified genes which don't exist in nature, and thus increasing the genetic pool available to improvement programs. Given this context, this project aims to comply with the wheat genetic transformation protocol in the hopes of obtaining in the future transgenic plants that are more adapted to the Brazilian Cerrado, supplying options to facilitate the cultivation in potential areas located in that region. For such, the response of eight cultivars will be tested, showing different tolerance levels to hydric stress, and one control cultivar, to two somatic embryogenesis induction protocols from immature embryos and to two protocols from isolated microspores. Cytological and histological studies will be held in order to confirm embryogenic callus. The minimal dose of the phosphinothricin's selected agent needed to select transformed tissues will be determined. Consequentially, the transient and stable transformation efficiencies will be compared, via Agrobacterium tumefaciens, containing the vector with the gene which is responsible for the resistance to the selected agent. The transgene's expression stability and the segregation pattern will be evaluated by means of histochemical analysis and response to the selecting agents in T 1 offspring. The hopes are that this project's result will help obtaining wheat cultivars which are more adapted to Cerrado's cultivation conditions, the intent is to expand cultivated areas and consequently to facilitate auto-sufficiency in Brazilian cereal production.

Ecosystem: Cerrados Region

Status: Completed Start date: Fri Apr 01 00:00:00 GMT-03:00 2011 Conclusion date: Mon Mar 31 00:00:00 GMT-03:00 2014

Head Unit: Embrapa Wheat

Project leader: Elene Yamazaki Lau

Contact: elene.yamazaki-lau@embrapa.br