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Protoplast production from napier grass and pearl millet triploid hybrids. Author(s): TIMBÓ, A. L. de O.; DAVIDE, L. C.; PINTO, J. E. B. P.; PEREIRA, A. V. The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of... ... Repository: BDPA Publication year: 2010
Banana protoplasts: culture and its applications. Author(s): MATSUMOTO, K.; MONTE, D. de C.; TEIXEIRA, J. B.; HAICOUR, R.; DAVEY, M. R. Repository: BDPA Publication year: 2010
Genetic engineering of filamentous fungi: prospects for obtaining fourth-generation biological products. Author(s): OLIVEIRA, L. R.; GONÇALVES, A. R.; QUINTELA, E. D.; COLOGNESE, L.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de Filamentous fungi exhibit unparalleled potential as cell factories for protein production, owing to their adeptness in protein secretion and remarkable proficiency in post-translational modifications.... ... Repository: BDPA Publication year: 2024
Enabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol. Author(s): HERNANDES-LOPES, J.; PINTO, M. S.; VIEIRA, L. R.; MONTEIRO, P. B.; GERASIMOVA, S. V.; NONATO, J. V. A.; BRUNO, M. H. F.; VIKHOREV, A.; FERNANDES, F. R.; GERHARDT, I. R.; PAUWELS, L.; ARRUDA, P.; DANTE, R. A.; YASSITEPE, J. E. de C. T. Here, we report the successful GE of agronomically relevant tropical maize lines using a MR-based, Agrobacterium-mediated transformation protocol previously optimized for the B104 temperate inbred lin... ... Repository: BDPA Publication year: 2023
Depleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides. Author(s): COSTA, L. S. de L.; ALVES, N. S. de F.; PINTO, C. E. M.; FLORENTINO, L. H.; MELO, B. P. de; MOREIRA, V. J. V.; SÁ, M. E. L. de; ARRAES, F. B. M.; RECH FILHO, E. L.; MORGANTE, C. V.; SA, M. F. G. de In this study, we identified two uORFs in the 5'-UTR sequence of this gene. The entire 5' leader sequence was amplified, cloned, and site-directed mutated to delete its uORFs start codons (-ATG). The... ... Repository: BDPA Publication year: 2023
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. Author(s): PRADO, G. S.; ROCHA, D. C.; SANTOS, L. N. dos; CONTILIANI, D. F.; NOBILE, P. M.; MARTINATI-SCHENK, J. C.; PADILHA, L.; MALUF, M. P.; LUBINI, G.; PEREIRA, T. C.; MONTEIRO-VITORELLO, C. B.; CRESTE, S.; BOSCARIOL-CAMARGO, R. L.; TAKITA, M. A.; CRISTOFANI-YALY, M.; SOUZA, A. A. de Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it h... ... Repository: BDPA Publication year: 2023
Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. Author(s): LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Brevipalpus-transmitted viruses (BTVs) belong to the genera Dichorhavirus and Cilevirus and are the main causal agents of the citrus leprosis (CL) disease. In this report, we explored aspects related... ... Repository: BDPA Publication year: 2020
Construction of gateway binary vector for selection with bialaphos or carboxin and GFP expression in fungi. Author(s): LOBO, I. K. C.; HANADA, R. E.; SOUSA, N. R.; GASPAROTTO, L.; SOUZA Jr. M. T.; KEMA, G. H. J.; SILVA, G. F. da Genomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (k... ... Repository: BDPA Publication year: 2011

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