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Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP). Author(s): PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations suc... ... Repository: BDPA Publication year: 2021
Efficient one-step knockout by electroporation of ribonucleoproteins into zona-intact bovine embryos. Author(s): CAMARGO, L. S. de A.; OWEN, J. R.; VAN EENENNAAM, A. L.; ROSS, P. J. Somatic cell nuclear transfer or cytoplasm microinjection have been used to generate genome-edited farm animals; however, these methods have several drawbacks that reduce their efficiency. This study... ... Repository: BDPA Publication year: 2020
Investigating engineered ribonucleoprotein particles to improve oral RNAi delivery in crop insect pests. Author(s): GILLET, F. X.; GARCIA, R. A.; MACEDO, L. L. P.; ALBUQUERQUE, E. V. S.; SILVA, M. C. M.; GROSSI-DE-SA, M. F. Repository: BDPA Publication year: 2017
Edição de genoma via non-homologous end joining (NHEJ) e ribonucleoproteínas (RNP). Author(s): PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. Introdução. Mecanismos de reparo genético. Non-homologous end joining (NHEJ). Mecanismo bioquímico. Edição de Genomas via NHEJ - aplicações práticas. Modulação e otimização de promotores. Ribonucleopr... ... Repository: BDPA Publication year: 2020
CRISPR technology in plant genome editing: biotechnology applied to agriculture. Author(s): MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. Chapter 1: Introduction to genome editing in plants. Chapter 2: Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP). Chapter 3: Genome editing by CRISPR/Cas via homologou... ... Repository: BDPA Publication year: 2021
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. Author(s): PRADO, G. S.; ROCHA, D. C.; SANTOS, L. N. dos; CONTILIANI, D. F.; NOBILE, P. M.; MARTINATI-SCHENK, J. C.; PADILHA, L.; MALUF, M. P.; LUBINI, G.; PEREIRA, T. C.; MONTEIRO-VITORELLO, C. B.; CRESTE, S.; BOSCARIOL-CAMARGO, R. L.; TAKITA, M. A.; CRISTOFANI-YALY, M.; SOUZA, A. A. de Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it h... ... Repository: BDPA Publication year: 2023

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