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Author(s): BERNARDO, A. P. da S.; SILVA, A. C. M. da; FRANCISCO, V. C.; RIBEIRO, F. A.; NASSU, R. T.; CALKINS, C. R.; NASCIMENTO, M. da S.; PFLANZER, S. B. This study evaluated the effects of freezing, prior to and after dry aging, on the microbiological and physical-chemical quality of beef. Strip loins (n = 24) from 12 carcasses were assigned to four t... ... |
Author(s): MAIA, M. da S.; BICUDO, S. D.; RODELLO, L. Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress i... ... |
Author(s): OLIVARES, C.; FONSECA, J. F. da; SARAIVA, H.; ALFRADIQUE, V.; CÔRTES, L.; COSTA, L.; BRANDÃO, F.; SOUZA-FABJAN, J. M. The aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram... ... |
Author(s): BOTTA, D.; ARRUDA, R. P. de; WATANABE, Y. F.; BALIEIRO, J. C. de C.; ROMANELLO, N.; BARRETO, A. do N.; PANTOJA, M. H. de A.; GIRO, A.; CARVALHO, C. P. T. de; OLIVEIRA, A. de S.; GARCIA, A. R. Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n =... ... |
Author(s): OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develo... ... |
Author(s): SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; MENEGOTTO, M.; VALENTE, P. The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-pr... ... |
Author(s): ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted... ... |
Author(s): OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z. Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that... ... |
Author(s): OLIVARES, C. C. S.; ALFRADIQUE, V. A. P.; SOUZA FABJAN, J. M. G.; FONSECA, J. F. da; SARAIVA, H. F. R. A.; CÔRTES, L. R.; COSTA, L. C. da; BRANDÃO, F. Z. Abstract: The success of IVF and subsequent development of embryos are directly related to sperm selection and quality. This study aimed to evaluate the effect of different forces and time of centrifu... ... |
Author(s): OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z. Abstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection... ... |
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