The detection of metal ions in plant tissues is an analytical problem of biological relevance with an impact on plant production. These ions are present in the tissues of plants, and insufficient or excessive values can lead to toxicity and change in their physiology. One of the analytical strategies for the determination of metallic ions in tissues is the use of electrochemical biosensors, which allow quick, selective and sensitive responses. This project aims at the construction of a biosensor containing peptides as electrochemical recognition and detection elements for copper determination in coffee tissues. This technology can be extended to other plant products.