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 Author(s): MORGANTE, C. V.; ARRAES, F. B. M.; MOREIRA-PINTO, C. E; MELO, B. P.; SA, M. F. G. de This chapter discusses CRISPR technology using the dead Cas9 variant (CRISPR/ dCas9), emphasizing on its use for modulating gene expression in plants. The main strategies currently used are presented... ... |
 Author(s): MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. Chapter 1: Introduction to genome editing in plants. Chapter 2: Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP). Chapter 3: Genome editing by CRISPR/Cas via homologou... ... |
 Author(s): PRADO, G. S.; ROCHA, D. C.; SANTOS, L. N. dos; CONTILIANI, D. F.; NOBILE, P. M.; MARTINATI-SCHENK, J. C.; PADILHA, L.; MALUF, M. P.; LUBINI, G.; PEREIRA, T. C.; MONTEIRO-VITORELLO, C. B.; CRESTE, S.; BOSCARIOL-CAMARGO, R. L.; TAKITA, M. A.; CRISTOFANI-YALY, M.; SOUZA, A. A. de Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it h... ... |
 Author(s): LOPES FILHO, J. H.; SILVA, V. C. H. da; SANTOS, J. C. dos; DANTE, R. A.; GERHARDT, I. R.; YASSITEPE, J. E. de C. T.; FERNANDES, F. R. Present and future challenges of modern agriculture. Gene editing tools for precision crop breeding. Genome editing methodologies. Origin of the CRISPR/Cas technology. Overview of mechanisms and enzym... ... |
 Author(s): PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations suc... ... |
 Author(s): VANEGAS K. G.; RENDSVIG, J. K. H.; JARCZYSKA, Z. D.; CÔRTES, M. V. de C. B.; VAN ESCH, A. P.; MORERA-GÓMEZ, M.; CONTESINI, F. J.; MORTENSEN, U. H. The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property... ... |
 Author(s): OLIVEIRA, L. R.; GONÇALVES, A. R.; QUINTELA, E. D.; COLOGNESE, L.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de Filamentous fungi exhibit unparalleled potential as cell factories for protein production, owing to their adeptness in protein secretion and remarkable proficiency in post-translational modifications.... ... |
 Author(s): SILVA, A. M. V.; SOUZA, E. M. G.; MARIN, S. R. R.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M.
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 Author(s): MORGANTE, C. V.; ARRAES, F. B. M.; PINTO, C. E. M.; MELO, B. P. de; GROSSI-DE-SA, M. F. Este capítulo aborda a tecnologia CRISPR pelo uso da variante dead Cas9 (CRISPR/ dCas9), com ênfase no seu uso para a modulação da expressão gênica em plantas. São apresentadas e discutidas as princip... ... |
 Author(s): BEZERRA L. P. de A. F.; MELO, B. P. de; ARRAES, F. B. M.; MORGANTE, C. V.; LOURENCO, I. T.; DOMICIANO, G. P.; ANDRADE, R. V.; FONTES, E. P. B.; SÁ, M. F. G. de In this study, we applied two strategies to suppress senescence induced by the DCD-NRP circuit to increase drought tolerance. Firstly, we investigated the potential of CRISPR/dCas9 system fused with t... ... |
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